Induction of sfiA SOS function by peroxides using three different E. coli strains

• Published in: Toxicology letters Vol. 48; no. 3; p. 225
• Main Authors: Eder, E, Favre, A, Stichtmann, C, Deininger, C
• Format: Journal Article
• Language: English
• Published: Netherlands 01.09.1989
• Subjects: beta-Galactosidase - analysis; DNA - drug effects; DNA Repair - drug effects; Escherichia coli - drug effects; Paraquat - toxicity; Peroxides - toxicity; SOS Response (Genetics) - drug effects
• ISSN: 0378-4274
• DOI: 10.1016/0378-4274(89)90048-9

Five peroxides and two related compounds were tested for genotoxicity by the SOS Chromotest using 3 different E. coli strains (PQ37, PM21, GC4798). All tested hydroperoxides (hydrogen peroxide, tert-butylhydroperoxide, cumene hydroperoxide) were clearly positive in all strains. From a comparison of results obtained from the different strains it can be concluded that neither DNA lesions leading to the induction of excision repair nor covalent binding of radicals to DNA is responsible for the induction of sfiA-SOS function by hydroperoxides. Among the remaining compounds tested, only dibenzoylperoxide gave a clearly positive result in strain PQ37 whereas di-tert-butylperoxide and azobisisobutyronitrile showed only borderline activity. When using strains PM21 and GC4798, none of the latter compounds was positive. Paraquat was inactive in all strains.