Screening Differential Expressions of Defense-related Responses in Cold-treated ‘Kyoho’ and ‘Campbell Early’ Grapevines

• Published in: Weon'ye gwahag gi'sulji Vol. 31; no. 3; pp. 275 - 281
• Main Authors: Ahn, Soon Young, Kim, Seon Ae, Han, Jae Hyun, Kim, Seung Heui, Yun, Hae Keun
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 2013; 한국원예학회
• Subjects: 농학; real-time PCR; low temperature; Vitis; abiotic stress
• ISSN: 1226-8763; 2465-8588
• DOI: 10.7235/hort.2013.13012

Low temperature is one of the major environmental factors that affect productivity including reduced growth and budding of vines, and changes of metabolic processes in grape (Vitis spp.). To screen the specific expression of abiotic stress-related genes against cold treatment in ‘Kyoho’ and ‘Campbell Early’ grapevines,expression of various defense-related genes was investigated by RT-PCR and real-time PCR. Among the 67 genes analyzed by RT-PCR and real-time PCR, 17 and 16 types of cDNA were up-regulated, while 5and 6 types were down-regulated in cold-treated ‘Kyoho’ and ‘Campbell Early’ grapevines, respectively. Genes encoding carotene (Cart3564 and Cart4472), chalcone isomerase (CHI), cytochrome P450 (CYP), flavonol synthase (FLS), endo-β-glucanase precursor (Glu), glutathione peroxidase (GPX), glutathione-S-transferase (GST), leucine-rich repeats (LRR), manganese superoxide dismutase (Mn-SOD), phenylalanine ammonia lyase (PAL), polygalacturonase-inhibiting protein (PGIP), proline rich protein 2 (PRP2), small heat shock protein (sHSP), temperature induced lipocalin (TIL), and thaumatin-like protein (TLP) were up-regulated, while those encoding CBF like transcription factor (CBF1), chitinase-like protein (CLP), cold induced protein (CIP),glycerol-3-phosphate acyltransferase (GPAT), and mitogen-activated protein kinase (MAPK) were down-regulated by low temperature treatment in both in ‘Kyoho’ and ‘Campbell Early’. KCI Citation Count: 0