Local release of monoamines in the gastrointestinal tract: an in vivo study in rabbits

Dialysis fibers chronically implanted into the gastric submucosa of rabbits allowed us to collect an interstitial fluid (I.S.F.) dialysate in which biogenic amine concentrations were measured, and compared with those obtained from plasma and tissue samples. The results suggest that I.S.F. concentrat...

Full description

Saved in:
Bibliographic Details
Published inLife sciences (1973) Vol. 38; no. 9; p. 827
Main Authors Meirieu, O, Pairet, M, Sutra, J F, Ruckebusch, M
Format Journal Article
LanguageEnglish
Published Netherlands 03.03.1986
Subjects
3,4-Dihydroxyphenylacetic Acid - analysis
Acetylcholine - pharmacology
Animals
Biogenic Amines - analysis
Chromatography, High Pressure Liquid
Dialysis
Digestive System - drug effects
Digestive System - metabolism
Dopamine - analysis
Epinephrine - analysis
Hydroxyindoleacetic Acid - analysis
Methoxyhydroxyphenylglycol - analysis
Neostigmine - pharmacology
Norepinephrine - analysis
Rabbits
Serotonin - analysis
Online AccessGet more information

Cover

More Information
Summary:Dialysis fibers chronically implanted into the gastric submucosa of rabbits allowed us to collect an interstitial fluid (I.S.F.) dialysate in which biogenic amine concentrations were measured, and compared with those obtained from plasma and tissue samples. The results suggest that I.S.F. concentrations represent a good assessment of the local release of the amines by enteric nerves and/or paracrine cells, under basal conditions. The fact that acetylcholine and neostigmine, when perfused through the dialysis system, increased I.S.F. serotonin (5-HT) concentrations, supports a cholinergic modulation of the release of 5-HT within the gastrointestinal wall, and validates the dialysis method as a powerful tool to monitor, in vivo, dynamic changes in I.S.F. monoamine concentrations.
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(86)90599-0