Incorporation of labeled 2,4,5,2',4',5'-hexachlorobiphenyl into the nuclear fraction of rat hepatocytes in vivo

2,4,5,2',4',5'-[14C] Hexachlorobiphenyl (2,4,5-HCB) a slowly metabolized PCB was given to rats by gastric incubation. The hepatocyte nuclei (HCN) were then isolated and treated with specific hydrolytic enzymes in order to separate the nucleic macromolecules, protein RNA and DNA. The r...

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Bibliographic Details
Published inToxicology (Amsterdam) Vol. 31; no. 3-4; p. 315
Main Authors Daubeze, M, Narbonne, J F
Format Journal Article
LanguageEnglish
Published Ireland 01.06.1984
Subjects
Animals
Carbon Radioisotopes
Cell Nucleus - metabolism
DNA - biosynthesis
Liver - metabolism
Polychlorinated Biphenyls - metabolism
Rats
RNA - biosynthesis
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Summary:2,4,5,2',4',5'-[14C] Hexachlorobiphenyl (2,4,5-HCB) a slowly metabolized PCB was given to rats by gastric incubation. The hepatocyte nuclei (HCN) were then isolated and treated with specific hydrolytic enzymes in order to separate the nucleic macromolecules, protein RNA and DNA. The results show that 2,4,5-HCB binds in vivo to hepatocyte nuclei. Liver nuclear proteins bind 70% of 2,4,5-HCB and 30% is found in the DNA fraction. No radioactivity was found in the nuclear RNA fraction at this experimental time (16 h).
ISSN:0300-483X
DOI:10.1016/0300-483X(84)90112-4