Two open reading frames adjacent to the Escherichia coli K-12 transketolase ( tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various Gram-positive bacteria

I have subcloned and sequenced the genes cmtB and cmtA of Escherichia coli K-12 which lie adjacent to the tkt gene on the chromosome. The genes cmtB and cmtA could encode a cytoplasmic protein (EIIA) and an integral-membrane protein (EIIBC), respectively, of the bacterial phospho enolpyruvate-depend...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 1158; no. 1; pp. 103 - 106
Main Author Sprenger, Georg A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 20.08.1993
Elsevier
Subjects
Amino Acid Sequence
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Bacterial
Enzyme IICB/enzyme IIA
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli K-12
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Microbiology
Molecular Sequence Data
Monosaccharide Transport Proteins
Open Reading Frames
PEP-dependent mannitol
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
phosphotransferase system
Restriction Mapping
Sequence Homology, Amino Acid
Staphylococcus - enzymology
Staphylococcus - genetics
Transketolase - genetics
Transport
Enzyme IICB/enzyme IIA
Transport
Escherichia coli K-12
PEP-dependent mannitol
phosphotransferase system
Nucleotide sequence
Enzyme
Escherichia coli
Sequence alignment
Bacteria
Phosphoenolpyruvate-dependent phosphotransferase system
Aminoacid sequence
Enterobacteriaceae
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Summary:I have subcloned and sequenced the genes cmtB and cmtA of Escherichia coli K-12 which lie adjacent to the tkt gene on the chromosome. The genes cmtB and cmtA could encode a cytoplasmic protein (EIIA) and an integral-membrane protein (EIIBC), respectively, of the bacterial phospho enolpyruvate-dependent carbohydrate phosphotransferase system. High similarity to the Enzymes II Mtl of Escherichia coli K-12 (gene mtlA) and of Staphylococcus carnosus was detected, but the two genes did not complement mannitol-negative E. coli mutants without the use of a heterologous promoter.
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(93)90103-F