Efficacy and Persistence of Entomopathogenic Fungi against Rhynchophorus ferrugineus on Date Palm: Host to Host Transmission

• Published in: Agronomy (Basel) Vol. 14; no. 4; p. 642
• Main Authors: Alwaneen, Waleed S., Wakil, Waqas, Kavallieratos, Nickolas G., Qayyum, Mirza Abdul, Tahir, Muhammad, Rasool, Khawaja G., Husain, Mureed, Aldawood, Abdulrahman S., Shapiro-Ilan, David
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.04.2024
• Subjects: Adults; Bioassays; biological control; Boxes; Cadavers; Conidia; Disease transmission; Eggs; Entomopathogenic fungi; Environmental impact; Fecundity; Females; Fungi; horizontal transmission; Insecticides; Insects; Laboratories; Larvae; Males; Mortality; mycosis; Oviposition; Pathogenicity; Pathogens; Pests; Plant resistance; red palm weevil; Rhynchophorus ferrugineus; sublethal effects; Sugarcane; Survival; Virulence; United States > US; Pakistan; Africa
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy14040642

The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae), is a destructive and voracious pest of palm species worldwide. Due to environmental and regulatory concerns, ecologically safe alternatives to synthetic chemical insecticides are needed to manage this cryptic insect species. Entomopathogenic fungi have the potential to manage this pest. The scope of management and effectiveness can be improved by direct control or horizontal transmission of entomopathogenic fungal isolates. We tested in the laboratory the virulence and pathogenicity of fifteen different entomopathogenic fungal isolates belonging to the following species: Beauveria bassiana, Metarhizium anisopliae, Beauveria brongniartii and Purpureocillium lilacinum. All fungal isolates were found virulent against larvae (14.9 ± 1.06 to 81.5 ± 1.48% mortality) and adults (5.6 ± 1.12 to 51.7 ± 1.51% mortality) at 12 d post-treatment. From a screening bioassay, five M. anisopliae (WG-08, WG-09) and B. bassiana (WG-23, WG-24, WG-25) isolates were tested for their concentration response mortality against larvae and adults after 7, 14 and 21 days (d) of treatment. Mortality was found positively correlated with concentration and time. At 21 d of treatment, WG-23 and WG-25 1 × 108 conidia/mL resulted in 100% mortality against larvae while only WG-25 1 × 109 conidia/mL caused 100% mortality of adults. Along with mortality, all the potential isolates have strong ovicidal effects that reduced 81.49% at 1 × 108 conidia/mL. The horizontal transmission bioassay indicated that the infected adults transmitted the disease to healthy individuals. Horizontal transmission of fungi from infected to non-infected adults not only caused significant mortality but also had a serious sublethal impact on insect development and fitness including reduced number of eggs/d fecundity, egg viability and neonate survival. Isolate WG-25 reduced oviposition (0.5 eggs/d), fecundity (11.7 eggs/female), egg viability (11.6%) along with larval survival 25.9% when infected male mated with normal female. In semi-field trials, all fungal isolates reduced survival of larvae found inside the palms and ultimately reduced infestations over a period of two months. The results of this study indicate that entomopathogenic fungi should be further tested for sustainable and efficient control of RPW in date palm production systems.