Levuglandin E2 inhibits mitosis and microtubule assembly

• Published in: Prostaglandins Vol. 39; no. 6; pp. 611 - 622
• Main Authors: MURTHI, K. K, SALOMON, R. G, STERNLICHT, H
• Format: Journal Article
• Language: English
• Published: Stoneham, MA Butterworth-Heinemann 01.06.1990
• Subjects: Animals; Arachidonic Acid; Arachidonic Acids - pharmacology; Biological and medical sciences; Brain - ultrastructure; Cattle; Cell Division - drug effects; Dinoprostone - pharmacology; Echinodermata; Fundamental and applied biological sciences. Psychology; Invertebrates; Kinetics; Microtubule Proteins - isolation & purification; Microtubule Proteins - metabolism; Microtubules - drug effects; Microtubules - ultrastructure; Mitosis - drug effects; Oocytes - cytology; Oocytes - drug effects; Prostaglandins E - metabolism; Prostaglandins E - pharmacology; Protein Binding; Sea Urchins; Prostaglandin; Echinodermata; Gel electrophoresis; Mitosis; Arachidonic acid derivatives; Prostaglandin H2; Arbacia punctulata; Marine environment; Cytoskeleton; Echinoidea; Microtubule associated protein; Inhibition; Microtubule; Invertebrata; Mechanism of action
• ISSN: 0090-6980
• DOI: 10.1016/0090-6980(90)90022-N

Levuglandin E2 (LGE2) is a gamma-keto aldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis. We show that exogenous LGE2 enters cells and efficiently inhibits the first synchronous cell division of fertilized sea urchin eggs. We attribute this inhibition to covalent modification of tubulin and thereby to inhibition of microtubule assembly.