Interferon-γ inhibition suppresses experimental allergic neuritis: modulation of major histocompatibility complex expression on Schwann cells in vitro

• Published in: Journal of neuroimmunology Vol. 31; no. 2; pp. 133 - 145
• Main Authors: Tsai, C.P., Pollard, J.D., Armati, P.J.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.02.1991; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Cell Aggregation; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class II - immunology; Immunosuppressive Agents - pharmacology; Inhibition of interferon-γ; Interferon-gamma - antagonists & inhibitors; Interferon-γ; Major Histocompatibility Complex - immunology; Major histocompatibility complex expression; Medical sciences; Nervous system involvement in other diseases. Miscellaneous; Neuritis, Autoimmune, Experimental - physiopathology; Neurology; P 2-responsive T cell; Schwann cell; Schwann Cells - immunology; T-Lymphocytes - physiology; Schwann cell; Interferon-γ; Inhibition of interferon-γ; Major histocompatibility complex expression; P 2-responsive T cell; Immunopathology; Animal model; Nervous system diseases; Rat; Induction; Major histocompatibility system; Rodentia; Class II histocompatibility antigen; Cytokine; Autoimmune disease; B-Lymphocyte; Nevritis; H-2-System; Vertebrata; Experimental disease; Mammalia; Antagonist; Class I histocompatibility antigen; Gamma interferon
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(91)90019-4

This study examines the modulation of major histocompatibility complex (MHC) expression on Lewis rat Schwann cells (Scs) cultured in the presence of dorsal root ganglion neurons (DRG). MHC class I and II molecules were induced on Scs using recombinant murine interferon-γ (IFN-γ), lymph node cells (LNC), removed at day 9 from Lewis rats with experimental allergic neuritis (EAN) and syngeneic T-cell line cells responsive to P 2 basic protein. EAN LNC induced MHC class I on Scs but only IFN-γ or P 2-responsive T-cells induced MHC class II. Control LNC from animals injected with Freund's adjuvant alone or naive spleen cells did not induce MHC class II. P 2 T-cells clustered in aggregates to the Scs. Similar studies were performed with inhibitors of IFN-γ; hydrocortisone, cyclosporin A, dibutyryl cyclic AMP, methyl-xanthine and prostaglandin E 2. Each agent produced a dose-dependent inhibition of MHC expression and prevented clustering of P 2-responsive T-cells to Scs.