Antimicrobial activity and biocompatibility of Ag+- and Cu2+-doped biphasic hydroxyapatite/α-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+-doped hydroxyapatite

• Published in: Applied surface science Vol. 307; pp. 513 - 519
• Main Authors: RADOVANOVIC, Zeljko, JOKIC, Bojan, VELJOVIC, Djordje, DIMITRIJEVIC, Suzana, KOJIC, Vesna, PETROVIC, Rada, JANACKOVIC, Djordje
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2014; Elsevier
• Subjects: Ag+-doped; Antiinfectives and antibacterials; Antimicrobial; Atomic absorption analysis; Biocompatibility; Candida albicans; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Cu2+-doped; Escherichia coli; Exact sciences and technology; HAp/α-TCP; Hydroxyapatite; In vitro testing; Phosphates; Physics; Pseudomonas aeruginosa; Spectroscopy; Staphylococcus aureus; Surgical implants; Antimicrobial; Ag+-doped; Biocompatibility; HAp/α-TCP; Cu2+-doped; Phosphates; doped; Cu; Ag; Doped materials; Hydroxyapatite
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2014.04.066

•Ag+-HAp and Cu2+-HAp powders were synthesized by a hydrothermal method.•Doped HAp powders were annealed and biphasic HAp/α-TCP doped with Ag+ or Cu2+ were obtained.•Doped HAp and HAp/α-TCP powders showed good antimicrobial activity against Staphylococcus aureus; Escherichia coli; Pseudomonas aeruginosa and Candida albicans.•In vitro MTT and DET tests (MRC-5 human fibroblast cells) showed good biocompatibility of doped HAp/α-TCP powders. Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200°C for 2h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/α-tricalcium phosphate (HAp/α-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/α-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energy-dispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. All powders showed good antimicrobial activity but generally the powders of doped HAp/α-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MTT and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/α-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/α-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/α-TCP as promising material for use in reconstructive surgery of bone.