Appraisal of rabies vaccine potency by determination of in vitro, specific interleukin-2 production

• Published in: Biologicals Vol. 19; no. 2; pp. 113 - 123
• Main Authors: Joffret, Marie-Line, Zanetti, Carlos, Morgeaux, Sylvie, Leclerc, Claude, Sureau, Pierre, Perrin, Pierre
• Format: Journal Article
• Language: English
• Published: Kent Elsevier Ltd 01.04.1991; Elsevier Science
• Subjects: Animals; Antibodies, Viral - biosynthesis; Bacteriology; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Interleukin-2 - biosynthesis; Lymphocytes - immunology; Mice; Mice, Inbred Strains; Microbiology; Neutralization Tests; Rabies - prevention & control; Rabies Vaccines - pharmacology; Rabies Vaccines - standards; rabies virus; Rats; Species Specificity; Vaccines, Inactivated - pharmacology; Vaccines, Inactivated - standards; Infection; Vertebrata; Mammalia; Mouse; Secretion; Viral disease; Interleukin; Rabies; Rodentia; Vaccine; Quantitative analysis
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1016/1045-1056(91)90009-9

The potency of different rabies vaccines was measured via cell mediated immunity (CMI) assessed by the production of interleukin-2 (IL-2) by CD4 +CD8 − lymphocytes. IL-2 production by splenocytes from mice immunized with various vaccines was measured following in vitro stimulation with antigens from different rabies and rabies-related strains. IL-2 production was specilic, reproducible and correlated with the vaccine protective activity as determined by the pre-exposure NIH test. Our results suggest that measurement of IL-2 production could be used for the appraisal of rabies vaccine potency.