Acetylcholine reduces net outward currents measured in vivo with single electrode voltage clamp techniques in neurons of the motor cortex of cats

Effects of acetylcholine (ACh) on membrane currents of cells of the motor cortex were measured directly, in vivo, in awake cats using single electrode voltage clamp (SEVC) techniques. Extracellular applications (90-95 nA) of 2 M ACh for periods of 30 s or less produced significant decreases in net o...

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Bibliographic Details
Published inBrain research Vol. 424; no. 1; p. 193
Main Authors Woody, C D, Gruen, E
Format Journal Article
LanguageEnglish
Published Netherlands 20.10.1987
Subjects
Acetylcholine - pharmacology
Animals
Cats
Electric Stimulation
Membrane Potentials - drug effects
Microelectrodes
Motor Cortex - drug effects
Motor Cortex - physiology
Neurons - drug effects
Neurons - physiology
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Summary:Effects of acetylcholine (ACh) on membrane currents of cells of the motor cortex were measured directly, in vivo, in awake cats using single electrode voltage clamp (SEVC) techniques. Extracellular applications (90-95 nA) of 2 M ACh for periods of 30 s or less produced significant decreases in net outward currents elicited by depolarizing commands whereas applications of saline did not. Reductions of net outward currents were also obtained after intracellular pressure injections of cyclic guanosine monophosphate (GMP)-dependent protein kinase (cGPK) mixed with 10 microM cyclic GMP.
ISSN:0006-8993
DOI:10.1016/0006-8993(87)91210-8