Metagenomic analysis of pioneer biofilm-forming marine bacteria with emphasis on Vibrio gigantis adhesion dynamics

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 217; p. 112619
• Main Authors: Gujinović, Luka, Maravić, Ana, Kalinić, Hrvoje, Dželalija, Mia, Šestanović, Stefanija, Zanchi, Dražen, Šamanić, Ivica
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2022; Elsevier
• Subjects: Adhesion dynamics; Biofilm formation; Condensed Matter; Marine biofilm composition; Physics; Pioneer biofilm bacteria; Soft Condensed Matter; Vibrio gigantis; Pioneer biofilm bacteria; Marine biofilm composition; Vibrio gigantis; Adhesion dynamics; Biofilm formation
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2022.112619

Marine biofilms occur frequently and spontaneously in seawater, on almost any submerged solid surface. At the early stages of colonization, it consists of bacteria and evolves into a more complex community. Using 16S rRNA amplicon sequencing and comparative metagenomics, the composition and predicted functional potential of one- to three-day old bacterial communities in surface biofilms were investigated and compared to that of seawater. This confirmed the autochthonous marine bacterium Vibrio gigantis as an early and very abundant biofilm colonizer, also functionally linked to the genes associated with cell motility, surface attachment, and communication via signaling molecules (quorum sensing), all crucial for biofilm formation. The dynamics of adhesion on a solid surface of V. gigantis alone was also monitored in controlled laboratory conditions, using a newly designed and easily implementable protocol. Resulting in a calculated percentage of bacteria-covered surface, a convincing tendency of spontaneous adhering was confirmed. From the multiple results, its quantified and reproducible adhesion dynamics will be used as a basis for future experiments involving surface modifications and coatings, with the goal of preventing adhesion. [Display omitted] •Vibrio gigantis is an abundant adherer in early phase of biofilm formation.•The essential biofilm forming genes and pathways are predicted in V. gigantis.•Adhesion dynamics of V. gigantis is quantified as surface coverage in bioreactor.•V. gigantis is a promising model for future antifouling research.