Morphological and histochemical characterization of the pectoral fin muscle of batoids

• Published in: Journal of morphology (1931) Vol. 284; no. 2; pp. e21548 - n/a
• Main Authors: Gabler‐Smith, Molly K., Coughlin, David J., Fish, Frank E.
• Format: Journal Article
• Language: English
• Published: United States 01.02.2023
• Subjects: Animal Fins - anatomy & histology; Animals; Biomechanical Phenomena; elasmobranch; Fishes; histology; locomotion; Locomotion - physiology; morphology; muscle fiber types; Muscle Fibers, Skeletal; Sharks; Skates, Fish - anatomy & histology; Swimming - physiology; muscle fiber types; elasmobranch; histology; morphology; locomotion
• ISSN: 0362-2525; 1097-4687
• DOI: 10.1002/jmor.21548

Batoids differ from other elasmobranch fishes in that they possess dorsoventrally flattened bodies with enlarged muscled pectoral fins. Most batoids also swim using either of two modes of locomotion: undulation or oscillation of the pectoral fins. In other elasmobranchs (e.g., sharks), the main locomotory muscle is located in the axial myotome; in contrast, the main locomotory muscle in batoids is found in the enlarged pectoral fins. The pectoral fin muscles of sharks have a simple structure, confined to the base of the fin; however, little to no data are available on the more complex musculature within the pectoral fins of batoids. Understanding the types of fibers and their arrangement within the pectoral fins may elucidate how batoid fishes are able to utilize such unique swimming modes. In the present study, histochemical methods including succinate dehydrogenase (SDH) and immunofluoresence were used to determine the different fiber types comprising these muscles in three batoid species: Atlantic stingray (Dasyatis sabina), ocellate river stingray (Potamotrygon motoro) and cownose ray (Rhinoptera bonasus). All three species had muscles comprised of two muscle fiber types (slow‐red and fast‐white). The undulatory species, D. sabina and P. motoro, had a larger proportion of fast‐white muscle fibers compared to the oscillatory species, R. bonasus. The muscle fiber sizes were similar between each species, though generally smaller compared to the axial musculature in other elasmobranch fishes. These results suggest that batoid locomotion can be distinguished using muscle fiber type proportions. Undulatory species are more benthic with fast‐white fibers allowing them to contract their muscles quickly, as a possible means of escape from potential predators. Oscillatory species are pelagic and are known to migrate long distances with muscles using slow‐red fibers to aid in sustained swimming. Batoids differ from other species of fish in that they possess dorsoventrally flattened bodies with enlarged muscled pectoral fins. Unlike other species of elasmobranchs, the main locomotory muscle in batoids is found in the enlarged pectoral fins. The locomotory muscles of sharks have a simple structure that are confined to the base of the fin; however, little to no data are available on the more complex musculature within the pectoral fins of batoids. Using gross morphological, histochemical and immunohistochemical analyses, we demonstrate differences in muscle fiber measurements between multiple locations across the pectoral fins of three species of batoid fishes.