Transference of natural diversity from the apomictic germplasm of Paspalum notatum to a sexual synthetic population

• Published in: Annals of applied biology Vol. 175; no. 1; pp. 18 - 28
• Main Authors: Zilli, Alex L., Acuña, Carlos A., Schulz, Roberto R., Marcón, Florencia, Brugnoli, Elsa A., Novo, Sabina F., Quarin, Camilo L., Martínez, Eric J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2019; Wiley Subscription Services, Inc
• Subjects: Agronomy; Breeding; Fertility; Genetic improvement; Genetic variability; Genotypes; Germplasm; Levels; Markers; microsatellite markers; Paspalum notatum; Ploidy; Population genetics; sexuality; Variability
• ISSN: 0003-4746; 1744-7348
• DOI: 10.1111/aab.12507

Genetic improvement in apomictic forage species has been restricted because of the absence of genetic variability in sexual germplasm with the same ploidy level. Following a new breeding scheme, a sexual synthetic tetraploid population (SSTP) of Paspalum notatum has been generated. The objectives of this work were: (a) to evaluate the genetic variability in SSTP by means of molecular markers, morphologic and agronomic traits, and seed fertility and quality traits and (b) to assess the transference of genetic variability from the apomictic germplasm to the sexual one. Molecular markers revealed a twofold higher level of variability in the SSTP in comparison with the sexual germplasm utilised for its generation, and similar levels with the apomictic ones; moreover, markers showed that most of the variability was inherited from the apomictic germplasm. Morphologic and agronomic traits and seed fertility and quality traits showed high levels of variation in the three groups of genotypes indicating that the new breeding scheme was effective in transferring variability from the apomictic germplasm to the SSTP. This new population will be useful in breeding of P. notatum, and the breeding scheme used for its generation may be used in other apomictic species. Genetic variability of a sexual synthetic tetraploid population (SSTP) of Paspalum notatum was evaluated by means of molecular markers, morphologic and agronomic traits, and seed fertility traits. Molecular markers revealed a twofold higher level of variability in the SSTP in comparison with the sexual germplasm utilised for its generation, and similar levels with the apomictic ones. Morphologic and agronomic traits and seed fertility traits showed high levels of variation in the three groups of genotypes indicating that the new breeding scheme was effective in transferring variability from the apomictic germplasm to the SSTP.