Molecular identification of blast resistance genes in rice landraces from northeastern India

Rice blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating diseases causing huge losses worldwide. In the present study, major blast resistance genes were investigated in landraces originating from northeastern India. Based on phenotypic evaluation, 288 landraces were...

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Bibliographic Details
Published inPlant pathology Vol. 68; no. 3; pp. 537 - 546
Main Authors Susan, A., Yadav, M. K., Kar, S., Aravindan, S., Ngangkham, U., Raghu, S., Prabhukarthikeyan, S. R., Keerthana, U., Mukherjee, S. C., Salam, J. L., Adak, T., Banerjee, A., Rath, P. C.
Format Journal Article
LanguageEnglish
Published Oxford Wiley Subscription Services, Inc 01.04.2019
Subjects
Association analysis
cluster analysis
Disease
Disease resistance
Fungi
Genes
Genotypes
Magnaporthe oryzae
Oryza
Phenotypic variations
Plant breeding
Population structure
Populations
resistance genes
Rice
Rice blast
Structural analysis
Variance analysis
India
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Summary:Rice blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating diseases causing huge losses worldwide. In the present study, major blast resistance genes were investigated in landraces originating from northeastern India. Based on phenotypic evaluation, 288 landraces were classified into three distinct groups: resistant (75), moderately resistant (127) and susceptible (86). The genetic frequencies of the 18 major blast resistance genes were between 6.2% and 27.4%, with only two genotypes possessing a maximum of nine blast resistance genes. The cluster and population structure analysis grouped the landraces into two groups. Through principal coordinate analysis, the scatter plots partitioned the resistant and moderately resistant landraces into different groups. Analysis of molecular variance showed maximum (96%) diversity within populations and least (4%) diversity between populations. Association analysis identified six markers, CRG4_2, RM72, tk59‐2, pi21_79‐3, RM1233 and RM6648, that are significantly associated with blast disease and explained a phenotypic variance of 1.1–6.5%. The associated genes could be used in marker‐assisted rice breeding programmes for gene pyramiding to develop rice varietal resistance against blast disease. The present study represents a valuable blast resistance genetic resource that could be used for identification of new R genes, donors for blast resistance, and genomic studies.
ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12975