DNA fragmentation in blue mussel (Mytilus edulis) sperm: aquaculture and fisheries implications

The objective of this study was to assess sperm DNA longevity in blue mussels (Mytilus edulis) using a dynamic assessment of sperm DNA fragmentation (SDF) after sperm activation. Mature blue mussels (n = 57) in Vigo (Galicia, Spain) were obtained, specifically rope farmed blue mussels (n = 38) and w...

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Bibliographic Details
Published inAquaculture research Vol. 48; no. 6; pp. 2973 - 2980
Main Authors Kjelland, Michael E, López‐Fernández, Carmen, Perez‐García, Cuca, Gosálvez, Jaime
Format Journal Article
LanguageEnglish
Published Oxford Hindawi Limited 01.06.2017
Subjects
Activation analysis
Aquaculture
Biological fertilization
Blue mussel (Mytilus edulis)
Damage
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
Fertilization
Fisheries
Fragmentation
Incubation period
Longevity
Low level
Marine molluscs
Mollusks
Mussels
Mytilus edulis
Rope
Seed production
Shellfish
Sperm
Spermatozoa
Spain
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Summary:The objective of this study was to assess sperm DNA longevity in blue mussels (Mytilus edulis) using a dynamic assessment of sperm DNA fragmentation (SDF) after sperm activation. Mature blue mussels (n = 57) in Vigo (Galicia, Spain) were obtained, specifically rope farmed blue mussels (n = 38) and wild blue mussels (n = 19). After the sperm collection, a subsample was assessed for SDF (0 h), while the rest of the sample was incubated for 6, 9, 12, 24 and 48 h at 15°C, assessing each time point using the Sperm‐Halomax kit (Halotech DNA, Madrid, Spain). The Kaplan–Meier estimator, log‐rank (Mantel–Cox) test and Mann–Whitney U‐test were used for statistical analyses (spss v. 16.0), α = 0.05. The rate of SDF (r‐SDF) between rope farmed and wild blue mussels over 0–6 h incubation was not significantly different (P = 0.278), but was for 6–24 h (P = 0.004). Differences in r‐SDF were observed when comparing the means between the two groups (P < 0.0001). Individual differences in r‐SDF existed among the rope farmed (P < 0.0001) and wild blue mussels (P < 0.0001). Wild blue mussels presented a higher DNA longevity than the farmed blue mussels. Selection of blue mussel males with a low level of sperm DNA damage and greater sperm DNA longevity may result in better fertilization and seed production.
ISSN:1355-557X
1365-2109
DOI:10.1111/are.13130