The effect of progesterone and human interferon α-2 on the release of PGF2α and PGE from epithelial cells of human proliferative endometrium

• Published in: Prostaglandins Vol. 44; no. 5; pp. 457 - 470
• Main Authors: MITCHELL, S. N, SMITH, S. K
• Format: Journal Article
• Language: English
• Published: Stoneham, MA Butterworth-Heinemann 01.11.1992
• Subjects: Arachidonic Acid - metabolism; Biological and medical sciences; Culture Techniques; Dinoprost - metabolism; Endometrium - metabolism; Epithelium - metabolism; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Humans; Interferon Type I - pharmacology; Mammalian female genital system; Progesterone - pharmacology; Prostaglandins E - metabolism; Recombinant Proteins; Time Factors; Vertebrates: reproduction; Human; Alpha interferon; Arachidonic acid derivatives; Cytokine; Prostaglandin E2; In vitro; Ovarian hormone; Prostaglandin F2α; Uterus; Eicosanoid; Female genital system; Progesterone; Sex steroid hormone; Endometrium
• ISSN: 0090-6980
• DOI: 10.1016/0090-6980(92)90140-O

Progesterone and interferon-like trophoblastic proteins modulate prostaglandin (PG) synthesis from endometrium in early ovine and bovine pregnancy. Enriched epithelial cells were prepared from human endometrium removed in the proliferative phase of menstrual cycle (n = 8). Progesterone at a concentration of 1 microM suppressed PGE release from the cells during the first 24 hours in culture. After 48 hours in culture progesterone at a dose of 100 nM and 1 microM suppressed both the release of PGF2 alpha and PGE from the cells and this suppression was maintained for a further two days. Addition of exogenous 30 microM arachidonic acid (AA) abolished this effect of progesterone on both PGF2 alpha and PGE release. Interferon alpha-2 did not suppress the basal release of PGF2 alpha nor PGE. In the presence of progesterone, interferon alpha-2 attenuated the progesterone mediated suppression of PGF2 alpha but not PGE release from endometrial cells. These findings suggest that progesterone suppresses the basal release of PGs from human endometrium, but unlike the sheep, interferon alpha-2 does not exert this action on human endometrium.