Effect of antiserum against aflatoxin B1-bovine serum albumin complex on aflatoxin B1-induced lysogenesis

Escherichia coli K12 bacteria lysogenic for the lambda phage were used to study the effect of antiserum against aflatoxin B1-induced lysogenesis. The antiserum was obtained from rabbits immunized with water in oil emulsion of aflatoxin B1-bovine serum albumin complex (AFB1-BSA). A marked reduction i...

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Bibliographic Details
Published inMutation research Vol. 209; no. 1-2; pp. 7 - 11
Main Authors Odugbesan, E A, Osowole, O A, Uwaifo, A O
Format Journal Article
LanguageEnglish
Published Netherlands 01.09.1988
Subjects
Aflatoxin B1
Aflatoxins - immunology
Aflatoxins - pharmacology
Antigen-Antibody Complex
Antigen-Antibody Reactions
Epoxy Compounds
Escherichia coli - genetics
Lysogeny - drug effects
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Summary:Escherichia coli K12 bacteria lysogenic for the lambda phage were used to study the effect of antiserum against aflatoxin B1-induced lysogenesis. The antiserum was obtained from rabbits immunized with water in oil emulsion of aflatoxin B1-bovine serum albumin complex (AFB1-BSA). A marked reduction in the degree of lysogenesis was observed when the antiserum was added to the reaction medium prior to microsomal enzyme activation of aflatoxin B1. There was no detectable effect when the antiserum was added after aflatoxin B1 activation. The result presented suggests that the antibodies in the AFB1-BSA antiserum can interact with aflatoxin B1 prior to its activation. This implies that an immune-protective effect can only be exerted if the antibodies intervene before activation.
ISSN:0027-5107
0165-7992
DOI:10.1016/0165-7992(88)90102-9