Prefrontal oxygenation is quantified with time-resolved near-infrared spectroscopy: effect of sex on baseline oxygenation and the response during exercise

• Published in: American journal of physiology. Regulatory, integrative and comparative physiology Vol. 325; no. 1; pp. R31 - R44
• Main Authors: Asahara, Ryota, Matsukawa, Kanji
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.07.2023
• Subjects: Cycles; Deoxygenation; Exercise - physiology; Female; Gender differences; Hemispheric laterality; Hemoglobin; Hemoglobins - metabolism; Humans; Infrared analysis; Infrared spectra; Infrared spectroscopy; Male; Men; Near infrared radiation; Optical properties; Oxygen; Oxygen Consumption - physiology; Oxygenation; Oxyhemoglobins - metabolism; Prefrontal cortex; Prefrontal Cortex - metabolism; Scattering coefficient; Sex; Sex differences; Spectroscopy, Near-Infrared - methods; Spectrum analysis; Women; prefrontal oxygenation; cycling exercise; baseline; time-resolved near-infrared spectroscopy; sex-related difference
• ISSN: 0363-6119; 1522-1490; 1522-1490
• DOI: 10.1152/ajpregu.00048.2023

With the use of time-resolved near-infrared spectroscopy that enables quantitative analysis of optical properties and oxygenation in cerebral tissues and thereby subject-to-subject comparisons in the variables, this study examined the influence of sex on baseline optical properties and oxygenation in the prefrontal cortex (PFC) and the responses during cycling exercise. Absolute levels of oxygenated- and deoxygenated-hemoglobin concentration (Oxy-Hb and Deoxy-Hb) in bilateral PFC were measured at rest and during unilateral cycling at low and moderate intensity in young participants (8 women and 10 men). Unilateral cycling was utilized to evaluate no lateralization of the prefrontal oxygenation responses during exercise. Baseline optical properties of bilateral PFC, defined optical path length and reduced scattering coefficient, and their responses during cycling were not different between the sexes. Baseline absolute Oxy-Hb of bilateral PFC was significantly lower in women (37 ± 3 μM) than in men (47 ± 7 μM), whereas absolute Deoxy-Hb revealed no sex-related difference. The absolute Oxy-Hb levels in bilateral PFC during low- and moderate-intensity cycling were also lower in women. However, no sex difference was observed when using changes against the baseline levels to normalize baseline differences. No laterality-related differences were observed in the changes in prefrontal Oxy-Hb and Deoxy-Hb during unilateral cycling. Ascertaining no sex-related difference in optical properties of the PFC, the current findings suggest that baseline absolute level of oxygenation in the PFC is lower in women than in men, likely due to lower oxygen supply rather than higher oxygen utilization, and that prefrontal oxygenation responds similarly during exercise independently of sex.