Disposable electrochemical aptasensor based on carbon nanotubes- V2O5-chitosan nanocomposite for detection of ciprofloxacin

• Published in: Sensors and actuators. B, Chemical Vol. 268; pp. 278 - 286
• Main Authors: Hu, Xiaobing, Goud, K. Yugender, Kumar, V. Sunil, Catanante, Gaëlle, Li, Zhanhong, Zhu, Zhigang, Marty, Jean Louis
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2018; Elsevier
• Subjects: Aptasensor; Chemical Sciences; Ciprofloxacin; Environmental Sciences; Life Sciences; MWCNTs; Screen printed carbon electrode; V2O5; MWCNTs; Ciprofloxacin; V2O5; Aptasensor; Screen printed carbon electrode
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2018.03.155

[Display omitted] •First reported a new type of electrochemical aptasensor was developed for detection of ciprofloxacin based on screen printed carbon electrode (SPCE) modified by carbon nanotube (CNT)-V2O5-chitosan (CS) nanocomposites.•ssDNA aptamer was successfully adsorbed on the CNT-V2O5-CS/SPCE basal electrode.•The developed electrochemical aptasensor exhibits good selectivity, stability and reproducibility, and lower limit of detection.•Practical application was demonstrated by milk sample. A novel electrochemical aptasensing transducer platform has been developed based on carbon nanotube (CNT)-V2O5-chitosan (CS) nanocomposites modified screen printed carbon electrode (SPCE) for the detection of ciprofloxacin. Using CNT-V2O5-CS/SPCE as the basal electrode transducer platform for the immobilization of the single-stranded DNA aptamer, and the electrochemical impedance spectroscopy (EIS) was selected for quantitative detection of ciprofloxacin (CIP). This aptasensor combines the biocompatibility of V2O5 nanoparticles, the efficient electron transfer capability of multiwalled CNTs, the effective film-forming strength of CS and the portability of SPCE. Under optimized experimental parameters, the developed aptasensor exhibited a dynamic range from 0.5 to 64.0 ng mL−1, where the linearity was between 0.5 to 8.0 ng mL−1 and the limit of detection (LOD) was 0.5 ng mL−1 of CIP. Furthermore, it showed good selectivity to CIP. For practical application, the aptasensor performance was verified in spiked milk samples and the acceptable recovery percentage of 94.50–97.87% (%RSD = 4.38, n = 3) was obtained.