Differential effects on RNA translation by a KCl extract of reticulocyte ribosomes: Characteristics of an inhibitory fraction

A KCl extract of rabbit reticulocyte ribosomes has been demonstrated to markedly stimulate the translation of various messenger RNAs in a cell-free system from Krebs II ascites tumor cells. In contrast, the translation of encephalomyocarditis viral RNA is strongly inhibited by the same extract. Frac...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 90; no. 3; pp. 824 - 831
Main Authors Stulberg, M.P., Isham, K.R.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 12.10.1979
Subjects
MOPC
EMC RNA
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Summary:A KCl extract of rabbit reticulocyte ribosomes has been demonstrated to markedly stimulate the translation of various messenger RNAs in a cell-free system from Krebs II ascites tumor cells. In contrast, the translation of encephalomyocarditis viral RNA is strongly inhibited by the same extract. Fractionation of the KCl extract allows the separation of these inhibitory and stimulatory activities. The inhibitory activity has been shown to be the consequence of an unusual endonuclease, associated with ribosomes, that produces approximately 4 S products from the degradation of globin mRNA and viral RNA.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(79)91902-8