Comparison of two rapid strip tests based on IGFBP-1 and PAMG-1 for the detection of amniotic fluid

• Published in: American journal of perinatology Vol. 25; no. 4; p. 243
• Main Authors: Chen, Frank Chih-Kang, Dudenhausen, Joachim Wolfram
• Format: Journal Article
• Language: English
• Published: United States 01.04.2008
• Subjects: Alpha-Globulins - analysis; Amniotic Fluid - chemistry; Biomarkers - analysis; Cesarean Section; Female; Fetal Membranes, Premature Rupture - diagnosis; Gestational Age; Humans; Immunoassay; Insulin-Like Growth Factor Binding Protein 1 - analysis; Placenta - metabolism; Pregnancy; Reagent Strips; Sensitivity and Specificity
• ISSN: 0735-1631; 1098-8785
• DOI: 10.1055/s-2008-1066876

Our objective was to compare two rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 (IGFBP-1) and of placental alpha-microglobulin-1 (PAMG-1). Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean section before delivery of the newborn. These samples were diluted with 0.9% saline solution in a dilution series down to concentrations of 1:320. Immunoassay strip tests were then compared in their ability to detect remaining concentrations of amniotic fluid. In 5 cases, both test methods showed the same results. In all remaining 15 cases, the test based on PAMG-1 proved to be superior by detecting amniotic fluid at least at one descending concentration below the test based on IGFBP-1. Thus, the rapid strip test based on PAMG-1 seems to be a more sensitive bedside test compared with the test based on IGFBP-1 for the detection of amniotic fluid.