Metabolism of prednisolone by the isolated perfused human placental lobule

Previous studies of the metabolism of 11 beta-hydroxy corticosteroids by placental tissue have indicated that the only product is the C11-oxidized metabolite. In the present study we have re-examined the metabolism of prednisolone in the isolated, perfused, dual recirculating human placental lobule,...

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Published inThe Journal of steroid biochemistry and molecular biology Vol. 39; no. 1; pp. 83 - 90
Main Authors ADDISON, R. S, MAGUIRE, D. J, MORTIMER, R. H, CANNELL, G. R
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Science 01.07.1991
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Gas
Chromatography, High Pressure Liquid
Female
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Humans
In Vitro Techniques
Maternal-Fetal Exchange
Other biological molecules
Perfusion
Placenta - metabolism
Prednisolone - metabolism
Pregnancy
Terpenes, steroids. Hormones
Human
Corticosteroid
Placenta
Metabolism
In vitro
Chromatography
Prednisolone
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Summary:Previous studies of the metabolism of 11 beta-hydroxy corticosteroids by placental tissue have indicated that the only product is the C11-oxidized metabolite. In the present study we have re-examined the metabolism of prednisolone in the isolated, perfused, dual recirculating human placental lobule, using a perfusate based on tissue culture medium 199. Four metabolites were identified in both the maternal and fetal compartments in 6 h perfusions by comparison of relative retention times measured by HPLC and capillary gas chromatography (GC) and of mass spectra recorded by capillary gas chromatography-mass spectrometry (GC-MS) with those of authentic reference standards. The steroids were derivatized as the MO-TMS ethers for mass spectral measurements. Analysis of samples from five perfusion experiments resulted in the following percentage conversions after 6 h perfusion (mean +/- SD, maternal and fetal perfusate, respectively): prednisone (49.1 +/- 7.8, 49.1 +/- 6.6), 20 alpha-dihydroprednisone (0.84 +/- 0.29, 0.81 +/- 0.35), 20 beta-dihydroprednisone (39.1 +/- 6.7, 39.2 +/- 5.9), 20 beta-dihydroprednisolone (6.8 +/- 2.7, 6.3 +/- 1.6) and unmetabolized prednisolone (4.1 +/- 1.8, 4.6 +/- 2.1). No evidence was found for metabolites formed by 6 beta-hydroxylation or cleavage of the C17-C20 bond.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0960-0760
1879-1220
DOI:10.1016/0960-0760(91)90016-X