Zebrafish Polycomb group gene ph2α is required for epiboly and tailbud formation acting downstream of FGF signaling
We analyzed Polycomb group gene ph2α functionally in zebrafish embryos by a gene knock-down procedure using morpholino antisense oligos. Inhibition of ph2α message translation resulted in abnormal epibolic movements as well as a thick tailbud or incomplete covering of the yolk plug. At the 24 hpf st...
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Published in | Biochemical and biophysical research communications Vol. 328; no. 4; pp. 858 - 866 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
25.03.2005
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Subjects | |
Online Access | Get full text |
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Summary: | We analyzed
Polycomb group gene
ph2α functionally in zebrafish embryos by a gene knock-down procedure using morpholino antisense oligos. Inhibition of
ph2α message translation resulted in abnormal epibolic movements as well as a thick tailbud or incomplete covering of the yolk plug. At the 24
hpf stage, morphants had short trunks and tails, phenotypes similar to those with disturbances in FGF signaling. Accordingly, we looked at the effects of
ph2α expression upstream and downstream of the FGF pathway. Treatment with SU5402, an inhibitor of Fgfrs, or injection of dominant-negative Fgfr1 DNA markedly reduced
ph2α expression in the tailbud. In addition, cells expressing mRNAs for
no tail,
spadetail,
myoD, and
papc, which are involved in FGF-related development of posterior mesoderm, were distributed abnormally. Collectively, the data argue that
ph2α is required for epiboly and tailbud formation, acting downstream of the FGF signaling pathway. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2005.01.031 |