Functions of the M-CSF receptor on osteoclasts

• Published in: Bone (New York, N.Y.) Vol. 18; no. 4; pp. 355 - 360
• Main Authors: YANG, S, ZHANG, Y, RODRIGUIZ, R. M, RIES, W. L, KEY, L. L
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 01.04.1996
• Subjects: Animals; Autoradiography; Binding, Competitive; Biological and medical sciences; Bone Resorption - chemically induced; Free Radicals - metabolism; Fundamental and applied biological sciences. Psychology; Humans; In Situ Hybridization; Iodine Radioisotopes; Isotope Labeling; Macrophage Colony-Stimulating Factor - metabolism; Macrophage Colony-Stimulating Factor - toxicity; Mice; Mice, Inbred C57BL; Osteoclasts - cytology; Osteoclasts - drug effects; Osteoclasts - metabolism; Phosphorylation; Radioligand Assay; Receptor, Macrophage Colony-Stimulating Factor - drug effects; Receptor, Macrophage Colony-Stimulating Factor - genetics; Receptor, Macrophage Colony-Stimulating Factor - metabolism; Recombinant Proteins - metabolism; Recombinant Proteins - toxicity; Skeleton and joints; Tyrosine - metabolism; Vertebrates: osteoarticular system, musculoskeletal system; Cytokine; Rodentia; Resorption; Osteoclast; Gene expression; Osteoarticular system; Vertebrata; Calvaria; Mammalia; Mouse; Newborn animal; Biological effect; Macrophage colony stimulating factor; Bone; Growth factor; Biological receptor
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/8756-3282(96)00013-0

Macrophage colony-stimulating factor (M-CSF) receptor has been previously reported to be present in osteoclasts both at mRNA and protein levels. However, the biochemical interactions between M-CSF and its receptor on osteoclasts are less well characterized than in mononuclear phagocytes. In this study, we show that (1) 125I-labeled M-CSF ligand specifically binds to the M-CSF receptor on osteoclasts by autoradiography; (2) binding of M-CSF to the receptor stimulates protein tyrosine phosphorylation in osteoclasts by immunostaining; (3) oxygen-derived free radicals produced by calvarial osteoclasts are increased by M-CSF stimulation (1.37 +/- 0.08, n = 10, P < 0.01); and (4) bone resorption in calvarial explants is enhanced by M-CSF (1.153 +/- 0.09, n = 10, p < 0.001). Thus, our data provide multiple lines of evidences that mouse calvarial osteoclasts are activated by M-CSF. These data suggest that under the conditions present in the calvarial model, M-CSF activates osteoclastic bone resorption.