Isolation of dimethylallylpyrophosphate: Tryptophan dimethylallyl transferase from the ergot fungus ( Claviceps spec.)

Cell-free extracts of Claviceps spec., strain SD 58, were obtained which catalyze the formation of L-dimethylallyltryptophan from dimethylallylpyrophosphate and L-tryptophan. The enzyme catalyzing this reaction has been partially characterized. It is absent from young mycelia, but begins to appear a...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 44; no. 5; pp. 1244 - 1251
Main Authors Heinstein, P.F., Lee, S.-I., Floss, H.G.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1971
Subjects
plant biochemistry
plant physiology
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Summary:Cell-free extracts of Claviceps spec., strain SD 58, were obtained which catalyze the formation of L-dimethylallyltryptophan from dimethylallylpyrophosphate and L-tryptophan. The enzyme catalyzing this reaction has been partially characterized. It is absent from young mycelia, but begins to appear at the end of the growth phase, preceding alkaloid formation by about one day.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(71)80219-X