In vitro clonal propagation of Gardenia latifolia Ait.: a toy making woody tree

• Published in: Agroforestry systems Vol. 87; no. 3; pp. 591 - 598
• Main Authors: Reddy, Y. Mohan, Saritha, K. V.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2013; Springer; Springer Nature B.V
• Subjects: Acclimatization; Agricultural biotechnology; Agriculture; Agronomy. Soil science and plant productions; Biological and medical sciences; Biomedical and Life Sciences; Forestry; Fundamental and applied biological sciences. Psychology; Gardenia; Gardens & gardening; General agronomy. Plant production; Genetics and breeding of economic plants; Haploidy, in vitro culture applications, somatic hybrids; Life Sciences; Plant breeding: fundamental aspects and methodology; Plant growth; Plant propagation; Plant reproduction; Shoots; Survival; Trees; Vegetative propagation. Micropropagation; Vegetative propagation. Sowing and planting. Harvesting; Vermicomposting; Plant regeneration; Nodal explant; Gardenia latifolia; Gardenia; Node; Explant; Micropropagation; Woody plant; Regeneration; Dicotyledones; Angiospermae; Rubiaceae; Tree; Spermatophyta; Clonal propagation; Agroforestry
• ISSN: 0167-4366; 1572-9680
• DOI: 10.1007/s10457-012-9579-4

An efficient, in vitro clonal propagation protocol has been established for Gardenia latifolia Ait. using mature nodal explants on Murashige and Skoog (MS) medium fortified with cytokinins (BA/Kn/2-iP) (1.0–5.0 mg l −1 ) in combination with auxin IAA (0.5 mg l −1 ). Maximum bud break (87 %) with shoot number (7.2 ± 0.26) observed on MS medium supplemented with BA (4.0 mg l −1 ) and IAA (0.5 mg l −1 ). Maximum number of shoots (30 ± 0.46) with shoot length of (0.9 ± 0.03 cm) observed on MS medium supplemented with BA (2.0 mg l −1 ), Kn (2.0 mg l −1 ) and IAA (0.5 mg l −1 ). Further elongation of shoots (3.5 ± 0.06 cm) was achieved on MS medium supplemented with BA (1.0 mg l −1 ) and IAA (0.1 mg l −1 ). About 70 % of root induction occurred in half-strength MS medium supplemented with IBA (4.0 mg l −1 ) in 4–6 weeks. Further elongation of roots with average length (9.0 cm) was achieved in culture bottles containing vermiculite and ¼ strength MS salts. After their partial hardening in these bottles for 30 days they were transferred to pots containing a mixture of soil and vermicompost (1:1) for acclimatization. The acclimatized plantlets were established in the field successfully with 85 % survival rate.