Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells

A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metaboli...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1928; p. 353
Main Authors Zhang, Jing, Zhang, Qing
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Animals
Biological Assay - instrumentation
Biological Assay - methods
Cell Line
Data Analysis
Energy Metabolism
Extracellular Space - metabolism
Glycolysis
Humans
Hydrogen-Ion Concentration
Mitochondria - metabolism
Neoplasms - metabolism
Oxidative Phosphorylation
Oxygen Consumption
Software
Glycolysis
Energy metabolism
Oxidative phosphorylation
ECAR
OCR
Seahorse XF24 Extracellular Flux analyzer
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Summary:A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9027-6_18