Dietary level of protein regulates glyceraldehyde-3-phosphate dehydrogenase content and synthesis rate in mouse liver cytosol

The content of liver cytosolic proteins was studied in mice subjected to protein depletion followed by refeeding with a normal diet. Depletion elicited either the accumulation or the decrease of several polypeptides, being the early increase of a Mr 36000 polypeptide the most pronounced change obser...

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Bibliographic Details
Published inMolecular and cellular biochemistry Vol. 115; no. 2; p. 117
Main Authors Sanllorenti, P.M. (Universidad Nacional de Mar del Plata, Mar del Plata, Argentina), Tardivo, D.B, Conde, R.D
Format Journal Article
LanguageEnglish
Published Netherlands 07.10.1992
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino Acid Sequence
Animals
CELLULE
CELULAS
Cytosol - drug effects
Cytosol - enzymology
DIETA
Dietary Proteins - pharmacology
Electrophoresis, Polyacrylamide Gel
Female
FOIE
Food Deprivation
GLICERALDEHIDO 3 FOSF DESHIDROG
GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO
Glyceraldehyde-3-Phosphate Dehydrogenases - biosynthesis
HIGADO
Liver - drug effects
Liver - enzymology
Methionine - metabolism
Mice
Mice, Inbred Strains
Molecular Sequence Data
PROTEINAS
PROTEINE
RATON
REGIME ALIMENTAIRE
Sequence Alignment
SOURIS
Sulfur Radioisotopes
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Summary:The content of liver cytosolic proteins was studied in mice subjected to protein depletion followed by refeeding with a normal diet. Depletion elicited either the accumulation or the decrease of several polypeptides, being the early increase of a Mr 36000 polypeptide the most pronounced change observed. The refeeding with a normal diet for 2 days caused a return of the cytosol protein composition to that of normally fed animals. The Mr 36000 polypeptide was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Its molecular weight, the sequence of its first twenty amino acid residues, its amino acid composition and its antigenic properties were found to be similar with those of GAPDH from different mammalian cells. During the first 2 days of protein depletion, both the GAPDH polypeptide content and activity increased. Thereafter, the enzymatic activity of GAPDH decreased, whereas GAPDH protein mass decreased in a lesser extent. The accumulation of GAPDH and other particular polypeptides in the cytosols of protein depleted mice was associated with an increased synthesis. The refeeding with a normal diet caused an immediate return to the synthesis pattern of normal livers
Bibliography:S20
9323132
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00230321