Litopenaeus vannamei Sma and Mad related protein 5 gene is involved in stress response and white spot syndrome virus infection

• Published in: Fish & shellfish immunology Vol. 117; pp. 104 - 112
• Main Authors: Chen, Yi-Hong, Lian, Yu-Ying, Lu, Ke-Cheng, Chen, Guo-Lian, Fan, Jin-Quan, Li, Bin-Bin, He, Jian-Guo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2021
• Subjects: Intracellular environment; Litopenaeus vannamei; Sma and Mad related protein 5; Unfolded protein response; White spot syndrome virus; Intracellular environment; Sma and Mad related protein 5; White spot syndrome virus; Litopenaeus vannamei; Unfolded protein response
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2021.07.021

Cell survival is based on the stability of intracellular state. It was well known that biochemical reactions in cells require specific intracellular environments, such as pH and calcium concentration. While the mechanism of stabilizing the intracellular environment is complex and far from clear. In this study, a Sma and Mad related protein 5 gene (LvSmad5) of Litopenaeus vannamei was cloned. LvSmad5 was located to both cytoplasm and nucleus. And subcellular localization of LvSmad5 was responsed to the changing of cells internal and external environment. Besides, it was found that subcellular localization of LvSmad5 was also regulated by unfolded protein response. Moreover, it was proved that nucleic localization of LvSmad5 could significantly increase the white spot syndrome virus (WSSV) infection in shrimp, and knockdown expression of LvSmad5 decreased the cumulative mortality of WSSV infection shrimp. Further investigation revealed that cytoplasm LvSmad5 could interplay with shrimp hexokinase 1, and contribute to glycolysis. These results indicated that LvSmad5 played a role in L. vannamei environmental stress response, and was used by WSSV for its replication. •Subcellular localization of L. vannamei Smad5 (LvSmad5) responsed to the internal or external environment stresses.•Nucleic localization of LvSmad5 was increased post WSSV infection.•Knockdown expression of LvSmad5 decreased the cumulative mortality of WSSV infection shrimp.•Cytoplasm LvSmad5 interplayed with L. vannamei hexokinase 1, and enhanced glycolysis.