Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis

• Published in: Plant cell, tissue and organ culture Vol. 86; no. 2; pp. 169 - 175
• Main Authors: Lin, C.S, Lai, Y.H, Sun, C.W, Liu, N.T, Tsay, H.S, Chang, W.C, Chen, J.J.W
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.08.2006
• Subjects: Bambusa; Biological and medical sciences; Biotechnology; chloroplast DNA; complementary DNA; Eukaryotic cell cultures; expressed sequence tags; Fundamental and applied biological sciences. Psychology; gene expression; genetic variation; hybridization; Methods. Procedures. Technologies; microarray technology; Miscellaneous; molecular sequence data; mutants; nucleotide sequences; photosynthesis; Phyllostachys edulis; plant breeding; Plant cells and fungal cells; Bambusa; Monocotyledones; Vegetals; Gramineae; Angiospermae; Spermatophyta; Identification; Hybridization; Mutation
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-006-9105-3

To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant. Ten expressed sequence tags (ESTs) were found to be differentially expressed; these were isolated and sequenced. RT-PCR analysis of these ESTs supported the results of the microarray analysis. Six ESTs that were nucleus-encoded exhibited differential expression patterns in the green wild-type bamboo relative to the albino mutant. These genes (exception being the Rubisco small subunit) were non-photosynthesis-related genes. The development of a specific SSH cDNA library in which most of the chloroplast-encoded or photosynthesis-related genes had been subtracted proved to be useful for studying the function of non-photosynthesis-related genes in the albino bamboo mutants with aberrant chloroplast genome. The combined use of this SSH library with microarray analysis will provide a powerful analytical tool for future studies of the bamboo genome.