LncRNA-NEAT1 promotes proliferation of T-ALL cells via miR-146b-5p/NOTCH1 signaling pathway

• Published in: Pathology, research and practice Vol. 216; no. 11; p. 153212
• Main Authors: Luo, Yun-Ya, Wang, Zhi-Hua, Yu, Qian, Yuan, Ling-Li, Peng, Hong-Ling, Xu, Yun-Xiao
• Format: Journal Article
• Language: English
• Published: Elsevier GmbH 01.11.2020
• Subjects: miR-146b-5p; NEAT1; NOTCH1; Proliferation; T-ALL; miR-146b-5p; NOTCH1; LncRNAs; Proliferation; NEAT1; T-ALL; GAPDH
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/j.prp.2020.153212

T-cell acute lymphoblastic leukemia (T-ALL) is a malignant tumor of the hematopoietic system, which can develop at any age, with the symptoms of weakness, fatigue, enlarged lymph nodes, or weight loss. Nuclear paraspeckle assembly transcript 1 (NEAT1) is involved in the process of T-ALL, but the regulatory mechanism is still not known clearly. The expression levels of NEAT1 and miR-146b-5p in T-ALL cells were performed by qRT-PCR and NOTCH1 protein level- wwWwas determined by western blot assay. Dual-luciferase reporter assay was used to detect the interaction between NEAT1 and miR-146b-5p, as well as miR-146b-5p and NOTCH1. The cell proliferation was measured by using MTT assay and colony formation assay. The expression levels of NEAT1 were markedly increased, but miR-146b-5p levels were reduced in T-ALL cells. Knockdown of NEAT1 or overexpression of miR-146b-5p decreased NOTCH1 expression, inhibited the proliferation of T-ALL cells. MiR-146b-5p bound both NEAT1 and NOTCH1 3′-UTR directly. Finally, inhibition of miR-146b-5p could abrogate the effects of NEAT1 knockdown on the proliferation of T-ALL cells. NEAT1 promotes the proliferation of T-ALL cells by sponging miR-146b-5p to upregulate the expression of NOTCH1. The results of this study provide new insight into the action mechanism of NEAT1 modulating T-ALL progression.