Transferability of microsatellite markers across six Dalbergia (Fabaceae) species and their characterization for Dalbergia miscolobium

In this study, 18 microsatellite markers previously developed and characterized for two Dalbergia species (Dalbergia nigra and Dalbergia monticola) were tested for six other species of the genus: Dalbergia decipularis, Dalbergia ecastaphyllum, Dalbergia elegans, Dalbergia frutescens, Dalbergia misco...

Full description

Saved in:
Bibliographic Details
Published inBiochemical systematics and ecology Vol. 69; pp. 161 - 165
Main Authors Buzatti, Renata Santiago de Oliveira, Chicata, F.S.L., Lovato, Maria Bernadete
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.12.2016
Subjects
alleles
Brazil
Dalbergia
Dalbergia ecastaphyllum
Dalbergia frutescens
Dalbergia miscolobium
Dalbergia nigra
Dalbergia sissoo
genetic markers
heterozygosity
loci
microsatellite
polymerase chain reaction
population genetics
SSR
Transferability
Brazil
microsatellite
SSR
Transferability
Dalbergia
Online AccessGet full text

Cover

More Information
Summary:In this study, 18 microsatellite markers previously developed and characterized for two Dalbergia species (Dalbergia nigra and Dalbergia monticola) were tested for six other species of the genus: Dalbergia decipularis, Dalbergia ecastaphyllum, Dalbergia elegans, Dalbergia frutescens, Dalbergia miscolobium, and Dalbergia sissoo. All species except D. sissoo are native to Brazil, and some have high-quality timber. Among the tested loci, 14 showed successful amplification in at least two species, and polymerase chain reaction (PCR) conditions were optimized for each of 8–12 transferred markers. Nine polymorphic loci were characterized for 50 individuals of two population of D. miscolobium. The total number of alleles for each locus ranged from 4 to 13. The observed and expected heterozygosity (He and Ho) ranged from 0.111 to 1.000 and from 0.108 to 0.842, respectively. All markers transferred from D. nigra and D. monticola in this study can be used to perform future population genetics studies of this important genus. •Transferability of simple sequence repeat (SSR) markers was successful among Dalbergia species.•At least eight markers amplified in the six Dalbergia species were tested.•Nine polymorphic markers were transferred and characterized in Dalbergia miscolobium.•These markers are valuable tools for population genetic study of these species.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1978
1873-2925
DOI:10.1016/j.bse.2016.07.017