DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues

Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics,calling for effective molecular probes.Herein,we performed cell-based systematic evolution of ligands by exponential enrichment（cell-SELEX） to obtain specific recognition of human prostate cancer...

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Bibliographic Details
Published inChinese chemical letters Vol. 28; no. 6; pp. 1252 - 1257
Main Authors Huang, Zhi-Xiang, Xie, Qin, Guo, Qiu-Ping, Wang, Ke-Min, Meng, Xiang-Xian, Yuan, Bao-Yin, Wan, Jun, Chen, Yuan-Yuan
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.2017
Subjects
Aptamer
Cell-SELEX
Clinical tissues
PC–3M
Prostate cancer
Aptamer
Clinical tissues
PC–3M
Prostate cancer
Cell-SELEX
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Summary:Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics,calling for effective molecular probes.Herein,we performed cell-based systematic evolution of ligands by exponential enrichment（cell-SELEX） to obtain specific recognition of human prostate cancer cells PC-3M.Four aptamers were successfully obtained that can bind to target cells with high affinity and specificity.A 51-nt truncated sequence named Xq-2-C1 was identified after further elaborative analysis on the secondary structure.More importantly,the achieved aptamer Xq-2-C1 not only demonstrated excellent specific to target cells,but also revealed specific recognition to clinical prostate cancer tissue.The tissue imaging results showed that Xq-2-C1 had better recognition ratio for clinical prostate cancer tissue samples（85%） compared to the random sequence（9%）.These results demonstrate that these newly generated aptamers would furnish potential applications in the early diagnosis and clinical treatment of prostate cancer.
Bibliography:Aptamer Cell-SELEX Prostate cancer PC-3M Clinical tissues
11-2710/O6
Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics,calling for effective molecular probes.Herein,we performed cell-based systematic evolution of ligands by exponential enrichment（cell-SELEX） to obtain specific recognition of human prostate cancer cells PC-3M.Four aptamers were successfully obtained that can bind to target cells with high affinity and specificity.A 51-nt truncated sequence named Xq-2-C1 was identified after further elaborative analysis on the secondary structure.More importantly,the achieved aptamer Xq-2-C1 not only demonstrated excellent specific to target cells,but also revealed specific recognition to clinical prostate cancer tissue.The tissue imaging results showed that Xq-2-C1 had better recognition ratio for clinical prostate cancer tissue samples（85%） compared to the random sequence（9%）.These results demonstrate that these newly generated aptamers would furnish potential applications in the early diagnosis and clinical treatment of prostate cancer.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2017.01.002