Genomic structure of murine mitochondrial DNA polymerase-gamma

We have sequenced a genomic clone of the gene encoding the mouse mitochondrial DNA polymerase. The gene consists of 23 exons, which span approximately 13.2 kb, with exons ranging in size from 53 to 768 bp. All intron-exon boundaries conform to the GT-AG rule. By comparison with the human genomic seq...

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Bibliographic Details
Published inDNA and cell biology Vol. 19; no. 10; p. 601
Main Authors Mott, J L, Denniger, G, Zullo, S J, Zassenhaus, H P
Format Journal Article
LanguageEnglish
Published United States 01.10.2000
Subjects
Animals
Base Sequence
DNA Polymerase gamma
DNA-Directed DNA Polymerase - genetics
Genes - genetics
Introns
Mice
Mitochondria - enzymology
Molecular Sequence Data
Promoter Regions, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
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Summary:We have sequenced a genomic clone of the gene encoding the mouse mitochondrial DNA polymerase. The gene consists of 23 exons, which span approximately 13.2 kb, with exons ranging in size from 53 to 768 bp. All intron-exon boundaries conform to the GT-AG rule. By comparison with the human genomic sequence, we found remarkable conservation of the gene structure; the intron-exon borders are in almost identical locations for the 22 introns. The 5' upstream region contains approximately 300 bp of homology between the mouse and human sequences that presumably contain the promoter element. This region lacks any obvious TATA domain and is relatively GC rich, consistent with the housekeeping function of the mitochondrial DNA polymerase. Finally, within the 5' flanking region, both mouse and human genes have a region of 73 bp with high homology to the tRNA-Arg gene.
ISSN:1044-5498
DOI:10.1089/104454900750019353