Wood formation in in vitro propagated walnut shoots in relation with root formation and development

• Published in: Acta botanica gallica Vol. 151; no. 1; pp. 45 - 53
• Main Authors: Kevers, Claire, Bisbis, Badia, Crèvecoeur, Michèle, Gaspar, Thomas, Dommes, Jacques
• Format: Journal Article Web Resource
• Language: English
• Published: Châtenay-Malabry Taylor & Francis Group 01.03.2004; Société botanique de France; Soc Botanique France
• Subjects: auxin; auxine; Biological and medical sciences; Biotechnologie; Biotechnology; Cell physiology; Cells, cell elements: structure and function; enracinement; Fundamental and applied biological sciences. Psychology; Life sciences; lignification; Noyer; Plant physiology and development; rooting; Sciences du vivant; walnut; Lignin; Lignification; Xylem; Root; Growth; Development; Above ground plant part; Stem
• ISSN: 1253-8078; 2166-3408; 2166-3408
• DOI: 10.1080/12538078.2004.10516019

Lignification and xylem cell multiplication for wood formation were examined in in vitro propagated walnut shoot cuttings after transfer on an auxin- containing rooting medium for one week and subsequently during root development in vermiculite in the absence of growth regulators. Lignification in the shoot stems started immediately after the exogenous auxin treatment which implied changes in peroxidase activity and in free IAA levels. Sustained lignification required the completion of the following rooting phases. The lignin was exclusively located in xylem cells, the number of which increased with the number of developing roots. The mutual interactions between the aerial parts of the plants and their roots are discussed.