6-Gingerol prevents free transition metal Ion [Fe (Ii)] Induced free radicals mediated alterations by In vitro and Ndv growth in chicken eggs by In ovo

• Published in: Pharmacognosy Magazine Vol. 14; no. 55; pp. 167 - 174
• Main Authors: Subbaiah, Ganjikunta, Reddy, Kesireddy, JayavardhanaRao, Yagani, Shanmugam, Bhasha, Ravi, Sahukari, Shanmugam, Kondeti, Narasimha, Golla
• Format: Journal Article
• Language: English
• Published: London Medknow Publications and Media Pvt. Ltd 01.04.2018; Sage Publications Ltd
• Subjects: Alkaloids; Antioxidants; Chemical properties; Cytochrome; Deoxyribonucleic acid; Disease; DNA; DNA damage; Eggs; Free radical reactions; Free radicals; Ginger; Health aspects; Iron compounds; Laboratories; Lipid peroxidation; Lipids; Mortality; Newcastle disease; Oxidation; Oxidative stress; Pharmacognosy; Pharmacological research; Physiological aspects; Prevention; Studies; Viral infections
• ISSN: 0973-1296; 0976-4062
• DOI: 10.4103/pm.pm_387_17

Background: The study of free radicals and their reactions is implicated in the pathogenesis of various human diseases. Therefore, antioxidants treatment plays a pivotal role in preventing the free radical-induced diseases. Objective: The objectives of this study were to examine the protective role of 6-gingerol from free transition metal ion-induced alterations by in vitro and Newcastle disease virus (NDV) in chicken eggs by in ovo. Materials and Methods: In this study, lipid peroxidation, DNA sugar oxidation, chelation of ferrous ions, DNA fragmentation, Cytochrome c-oxidation, ferric ion reducing activities and DPPH, superoxide and hydroxyl radicals scavenging activity by ESR studies were examined with 6-gingerol by in vitro. Furthermore, antiviral activity was screened with 6-gingerol by in ovo. Results: In vitro results showed that 254 μM/ml of 6-gingerol firmly inhibited erythrocyte membrane lipid peroxidation by 85.28% and DNA sugar oxidation by 91.26% when compared with butylated hydroxyanisole 74.42% (554 μM/ml) and 78.30% (824 μM/ml), respectively. Whereas, chelating activity of 6-gingerol was 80.2% which nearly similar to the EDTA activity (50 μg/ml) shown 83.93%. In addition, 6-gingerol (254μM/ml) scavenged the superoxide, hydroxyl, DPPH radicals by 84.96%, 90.45%, and 94.63% respectively and the same was strongly supported with ESR studies. Further, 254 μM/ml of 6-gingerol inhibited the DNA fragmentation, Cytochrome c-oxidation and reducing the ferric ion by in vitro and antiviral activity shown on NDV at 100 μg/ml. Conclusion: This study could enlighten that the 6-gingerol has a good metal sequester property and it could prevent the free radicals by in vitro and NDV growth in chicken eggs by in ovo. Abbreviations used: NDV: Newcastle Disease Virus; ESR: Electron Spin resonance; SOD: Super oxide Dismutase; HA: Hemagglutination.