Synchronized spectrofluorimetric determination of ponatinib and curcumin as an effective therapeutic combination in laboratory prepared mixtures and human plasma samples

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 264; p. 120235
• Main Authors: El Sharkasy, Mona E., Aboshabana, Rasha, Belal, F., Walash, M., Tolba, Manar M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.01.2022
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2021.120235

[Display omitted] •This is the first report on synchronous spectrofluorimetric method for simultaneous determination of ponatinib and curcumin.•Determination of the analytes in spiked human plasma with high recoveries.•The proposed method was fully validated in compliance with ICH guidelines. Curcumin is a natural product that is frequently utilized in cancer prevention and treatment. The significant benefit of vegetable-derived nutraceuticals in combination with widespread cytostatic medication such as ponatinib is to reduce toxicity and side effects. In this paper, we focus the study on analytical quantification of ponatinib and curcumin through highly sensitive synchronous spectrofluorometric method. Applying this method at Δλ = 160 nm, each of ponatinib and curcumin could be measured at 303 and 412 nm without interference from each others. The diverse experimental factors impacting the performance of the method were studied and optimized. The method exhibited a reasonable linearity in the ranges of 5.0–60.0 and 10.0–200.0 ng/mL for ponatinib and curcumin, respectively with detection limits of 1.48 and 1.22 ng/mL and quantitation limits of 4.49 and 3.68 ng/mL, respectively. The anticipated method was employed for the assessment and evaluation of the studied drugs in the spiked human plasma samples. The mean % recoveries in plasma samples (n = 6) for each of ponatinib and curcumin were 99.84 ± 1.86 and 100.06 ± 2.72, accordingly. The developed method was validated in conformity with the requirements of International Council of Harmonization (ICH).