A simple colorimetric and fluorometric probe for rapid detection of CN– with large emission shift
[Display omitted] •A simple developed fluorescent probe was synthesized for rapid detection of CN– in colorimetric and fluorometric signal channels.•The crystal structure of the probe is reported.•The probe works in DMSO/HEPES (3:7, v/v) solution with large fluorescent emission (595 nm) and large St...
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Published in | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 280; p. 121540 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
05.11.2022
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
•A simple developed fluorescent probe was synthesized for rapid detection of CN– in colorimetric and fluorometric signal channels.•The crystal structure of the probe is reported.•The probe works in DMSO/HEPES (3:7, v/v) solution with large fluorescent emission (595 nm) and large Stokes shifts (145 nm).•The probe has been successfully applied to the imaging of CN− in C6 cells.
In this work, a novel probe R was synthesized via Knoevenagel reaction between 3H-benzo[f]chromium-2-formaldehyde and ethyl cyanoacetate for selective detection of CN– in both colorimetric and fluorescent signal channels. The recognition of CN– was through the nucleophilic reaction of CN– to C = C of probe R, which destroys π-conjugation and blocks the ICT effect of the probe, resulting in colorimetric and fluorometric responses. Probe R showed great sensitivity toward CN–, with large fluorescent emission (595 nm) and low detection limit (0.70 μM). Moreover, probe R can detect exogenous CN– in living cells. |
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ISSN: | 1386-1425 |
DOI: | 10.1016/j.saa.2022.121540 |