Ultrasensitive mercury ion and biothiol detection based on Dansyl-His-Pro-Gly-Asp-NH2 fluorescent sensor
A novel Dansyl-labeled tetrapeptide sensor (D-P4) was designed for the ultrasensitive detection of mercury ion by spectroscopic analyses. The sensor exhibited an excellent selectivity and sensitivity to Hg2+ with a ‘turn-off’ fluorescence response. In addition, D-P4-Hg system presented a good ‘turn-...
Saved in:
Published in | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 250; p. 119246 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
05.04.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A novel Dansyl-labeled tetrapeptide sensor (D-P4) was designed for the ultrasensitive detection of mercury ion by spectroscopic analyses. The sensor exhibited an excellent selectivity and sensitivity to Hg2+ with a ‘turn-off’ fluorescence response. In addition, D-P4-Hg system presented a good ‘turn-on’ fluorescence response to biothiols. The research findings provide a strategy for the design of new sensors to detect metalions.
[Display omitted]
•Ultrasensitive Hg2+ and biothiol detection based on the fluorescent peptidyl sensor were developed.•Spectroscopic analyses were applied to study the biointeraction mechanism of Hg2+ to peptidyl sensor.•The possible binding site of Hg2+ to peptidyl sensor was predicted by molecular docking method.•The fluorescent sensor exhibits an excellent selectivity and sensitivity to Hg2+ with a ‘turn-off’ fluorescence response.
Mercury is an environmental contaminant, which is highly toxic even at extremely low concentrations. Long-term accumulation of mercury in human body will damage the central nervous system or digestive tract system. Here, a new fluorescent chemical sensor Dansyl-His-Pro-Gly-Asp-NH2 (D-P4) was synthesized for the determination of Hg2+. The D-P4 sensor exhibits excellent selectivity and sensitivity to Hg2+ in aqueous solution with a ‘turn-off’ fluorescence response. Furthermore, D-P4-Hg system displays a good ‘turn-on’ fluorescence response to biothiols. The calculated binding constant for the 1:1 complex of D-P4 with Hg2 + is 1.07 × 105 M−1, which also confirms the high affinity of D-P4 for Hg2+. Results indicate that the detection limit of D-P4 for Hg2+ is 61.0 nM, and that of D-P4-Hg system for Cys is as low as 80.0 nM. |
---|---|
ISSN: | 1386-1425 |
DOI: | 10.1016/j.saa.2020.119246 |