Methidium intercalator inserted into synthetic oligonucleotides

A new methidium intercalator phosphoramidite has been synthesized. Methidium incorporation into an oligonucleotide during the synthesis was confirmed by UV and MALDI TOF MS data. UV melting experiments showed enhanced stability of a duplex, containing internal methidium. Methidium phosphoramidite ha...

Full description

Saved in:
Bibliographic Details
Published inTetrahedron letters Vol. 37; no. 47; pp. 8467 - 8470
Main Authors Timofeev, Edward N., Smirnov, Igor P., Haff, Lawrence A., Tishchenko, Ekaterina I., Mirzabekov, Andrei D., Florentiev, Vladimir L.
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 18.11.1996
Elsevier
Subjects
BASIC BIOLOGICAL SCIENCES
Chemistry
Chemistry, Organic
CLATHRATES
OLIGONUCLEOTIDES
Physical Sciences
Science & Technology
STABILITY
SYNTHESIS
SYNTHETIC MATERIALS
BINDING
TRIPLE-HELIX FORMATION
SEQUENCE
Online AccessGet full text

Cover

More Information
Summary:A new methidium intercalator phosphoramidite has been synthesized. Methidium incorporation into an oligonucleotide during the synthesis was confirmed by UV and MALDI TOF MS data. UV melting experiments showed enhanced stability of a duplex, containing internal methidium. Methidium phosphoramidite has been synthesized and used for insertion of intercalator into the deoxyoligonucleotides.
Bibliography:DE-AC02-06CH11357
ANL/CMB/JA-23413
ISSN:0040-4039
1873-3581
DOI:10.1016/0040-4039(96)01968-5