High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum

• Published in: Plant cell, tissue and organ culture Vol. 79; no. 2; pp. 169 - 179
• Main Authors: Synkova, H, Semoradova, S, Burketova, L
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Dordrecht Springer 01.11.2004
• Subjects: abiotic stress; ascorbate peroxidase; Biological and medical sciences; Biotechnology; catalase; cytokinins; endogenous sources; enzyme activity; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; glutathione reductase (NADPH); Methods. Procedures. Technologies; Miscellaneous; Nicotiana tabacum; pathogenesis-related proteins; peroxidase; Plant cells and fungal cells; plant stress; protein synthesis; stress tolerance; tobacco; Vegetals; Pssu- ipt tobacco; antioxidant enzymes; Antioxidant; Nicotiana tabacum; Cytokinin; In vitro; enzymes of intermediary metabolism; Stress; Enzymatic activity; Dicotyledones; Angiospermae; Spermatophyta; Solanaceae; Intermediary metabolism; PR proteins; ex vitro
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-004-0657-9

Transgenic Pssu-ipt tobacco with elevated content of endogenous cytokinins grown under in vitro conditions exhibited elevated activities of antioxidant enzymes (i.e. catalase, ascorbate peroxidase, guaiacol and syringaldazine peroxidase, glutathione reductase) and some of enzymes involved in anaplerotic pathways such as glucose-6-phosphate dehydrogenase, glycolate oxidase, NADP-malic enzyme, NADP-isocitrate dehydrogenase, and glutamate dehydrogenase compared to control non-transgenic SR1 tobacco. Higher activities of peroxidases, NADP-malic enzyme, and glutamate dehydrogenase were maintained in transgenic grafts after several weeks of the growth under ex vitro conditions, while transgenic rooted plants showed only the increase in activity of glycolate oxidase compared to control non-transformed tobacco. Total activities of superoxide dismutase were lower in both types of Pssu-ipt tobacco contrary to controls under both growth conditions. The presence of PR-1 protein and proteins with elevated activities of chitinase was proved in the extracellular fluid in both transgenic types under both in vitro and ex vitro conditions.