Oxidative Protein Folding Promotion by Imidazoyl-conjugated Thiol

The promotion of protein folding is an important subject that can contribute to the efficient production of biological medicines. Here we report the promotion of disulfide bond-coupled protein folding by imidazoyl-conjugated thiol (ImdSH). ImdSH accelerated the disulfide bond formation and folding o...

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Bibliographic Details
Published inChemistry letters Vol. 52; no. 3; pp. 202 - 205
Main Authors Okada, Shunsuke, Matsumoto, Yosuke, Okumura, Masaki, Muraoka, Takahiro
Format Journal Article
LanguageEnglish
Japanese
Published OXFORD Oxford Univ Press 05.03.2023
Subjects
Chemistry
Chemistry, Multidisciplinary
Physical Sciences
Science & Technology
Thiol
DISULFIDE BOND
RIBONUCLEASE-A
REGENERATION
Folding
Protein
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Summary:The promotion of protein folding is an important subject that can contribute to the efficient production of biological medicines. Here we report the promotion of disulfide bond-coupled protein folding by imidazoyl-conjugated thiol (ImdSH). ImdSH accelerated the disulfide bond formation and folding of reduced ribonuclease A and bovine pancreatic trypsin inhibitor relative to glutathione as a conventionally used additive.
Bibliography:KAKEN
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.220537