Concentrations of anti-Müllerian hormone in fluid from small human antral follicles show a negative correlation with CYP19 mRNA expression in the corresponding granulosa cells

• Published in: Molecular human reproduction Vol. 16; no. 9; pp. 637 - 643
• Main Authors: Eilsø Nielsen, M., Rasmussen, I.A., Fukuda, M., Westergaard, L.G, Yding Andersen, C.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2010
• Subjects: Adolescent; Adult; AMH; AMH-R2; Androstenedione - analysis; Anti-Mullerian Hormone - analysis; Aromatase - genetics; CYP19; Estradiol - analysis; Female; Follicular Fluid - chemistry; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Granulosa Cells - enzymology; Humans; Inhibins - analysis; Progesterone - analysis; Receptors, FSH - genetics; Receptors, LH - genetics; Receptors, Peptide - genetics; Receptors, Transforming Growth Factor beta - genetics; RNA, Messenger - isolation & purification; small human antral follicles; steroids; Testosterone - analysis; Young Adult
• ISSN: 1360-9947; 1460-2407
• DOI: 10.1093/molehr/gaq001

Ovaries surgically removed for fertility preservation from a total of 24 women served as a source of human small antral follicles, including the follicular fluid (FF) and the corresponding granulosa cells (GC). The FF was used to evaluate the intrafollicular concentrations of anti-Müllerian hormone (AMH), inhibin-B, estradiol, progesterone, androstenedione and testosterone. In GC mRNA expression of the AMH type II receptor (AMH-r2) was determined and correlated to the mRNA expression of CYP19 (aromatase), FSH-receptor (FSH-r) and LH-receptor (LH-r) and to the hormonal profiles of the corresponding FF. GC and FF from a total of 64 follicles (diameter of 3–9 mm) were evaluated. Concentrations of AMH in FF showed a highly significant inverse correlation with CYP19 mRNA expression in the corresponding GC and with concentrations of estradiol, progesterone and inhibin-B in the FF. However, a small subgroup of follicles exhibited high levels of AMH simultaneously with relative high levels of CYP19 mRNA. In contrast to AMH, mRNA expression of AMH-r2 was significantly positively correlated to the mRNA expression of FSH-r and CYP 19, but failed to correlate to any other measured parameters. These data confirms an intimate correlation between follicular AMH levels, AMH-r2, FSH-r expression and estradiol secretion in the developing human follicle.