Gene expression of double-stranded RNA-dependent protein kinase in the human endometrium and decidua

To clarify the biological significance of double-stranded RNA-dependent protein kinase (PKR), an interferon (INF)-inducible substance, we investigated (1) PKR gene expression and the (2) effect of IFN-γ on PKR gene expression in human endometrium. By Northern blot analysis, PKR mRNA was detected as...

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Bibliographic Details
Published inMolecular and cellular endocrinology Vol. 158; no. 1; pp. 65 - 68
Main Authors Taga, Michiyoshi, Ikeda, Mario, Shigeta, Hiroyuki, Hiraga, Machiko
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 20.12.1999
Subjects
Blotting, Northern
Cells, Cultured
Decidua - cytology
Decidua - enzymology
eIF-2 Kinase - genetics
eIF-2 Kinase - metabolism
Endometrium
Endometrium - cytology
Endometrium - enzymology
Female
Gene Expression
Humans
INF
Interferon-gamma - pharmacology
PKR
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
INF
Gene expression
PKR
Endometrium
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Summary:To clarify the biological significance of double-stranded RNA-dependent protein kinase (PKR), an interferon (INF)-inducible substance, we investigated (1) PKR gene expression and the (2) effect of IFN-γ on PKR gene expression in human endometrium. By Northern blot analysis, PKR mRNA was detected as a 2.5 kb band in human endometrium throughout the menstrual cycle and decidua in early pregnancy. The addition of IFN-γ to culture medium increased the PKR mRNA level in a dose-dependent manner in cultured endometrial stromal cells. These results suggest that IFN-γ, which is reported to have an inhibitory effect on cell proliferation, plays an important role in human endometrial function by mediating PKR gene expression.
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(99)00182-3