Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney

• Published in: Inflammation research Vol. 58; no. 3; pp. 143 - 150
• Main Authors: Jung, K. J., Lee, E. K., Kim, J. Y., Zou, Y., Sung, B., Heo, H. S., Kim, M. K., Lee, J., Kim, N. D., Yu, B. Pal, Chung, H. Y.
• Format: Journal Article
• Language: English
• Published: Basel Birkhäuser-Verlag 01.03.2009; Springer Nature B.V
• Subjects: Aging - physiology; Allergology; Animals; Biomedical and Life Sciences; Biomedicine; Caloric Restriction; Dermatology; Diet; Immunology; Inflammation - immunology; Kidney - metabolism; Lipid Peroxidation; Male; Mitogen-Activated Protein Kinases - metabolism; Neurology; NF-kappa B p50 Subunit - metabolism; Oxidation-Reduction; Oxidative Stress; Pharmacology/Toxicology; Prostaglandin-Endoperoxide Synthases - metabolism; Rats; Rats, Inbred F344; Reactive Oxygen Species - metabolism; Rheumatology; Signal Transduction - physiology; Transcription Factor AP-1 - metabolism; Aging; Short term calorie restriction; Molecular inflammation; AP-1; NF-kB
• ISSN: 1023-3830; 1420-908X
• DOI: 10.1007/s00011-008-7227-2

. Objective: To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney. Treatment: Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5). Methods: Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot. Results: 10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1. Conclusions: We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.