Differentiation of THP-1 monocytes to macrophages increased mitochondrial DNA copy number but did not increase expression of mitochondrial respiratory proteins or mitochondrial transcription factor A

• Published in: Archives of biochemistry and biophysics Vol. 710; p. 108988
• Main Authors: Okamoto, Mizuho, Shimogishi, Masanori, Nakamura, Akari, Suga, Yusuke, Sugawara, Kyosuke, Sato, Michio, Nishi, Ryotaro, Fujisawa, Akio, Yamamoto, Yorihiro, Kashiba, Misato
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.10.2021
• Subjects: Coenzyme Q10; Macrophage; mtDNA copy number; PMA; TFAM; THP-1; THP-1; TFAM; Coenzyme Q10; PMA; mtDNA copy number; Macrophage
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2021.108988

Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions.