Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana

• Published in: Plant cell, tissue and organ culture Vol. 80; no. 3; pp. 239 - 246
• Main Authors: Mirici, S, Parmaksiz, I, Ozcan, S, Sancak, C, Uranbey, S, Sarihan, E.O, Gumuscu, A, Gurbuz, B, Arslan, N
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.03.2005
• Subjects: 2,4-D; benzyladenine; Biological and medical sciences; Biotechnology; bulbs; culture media; dose response; embryo (plant); embryogenesis; endangered species; Eukaryotic cell cultures; explants; Fundamental and applied biological sciences. Psychology; in vitro culture; in vitro regeneration; medicinal plants; methodology; Methods. Procedures. Technologies; micropropagation; Miscellaneous; Murashige and Skoog medium; naphthaleneacetic acid; organogenesis; Plant cells and fungal cells; Sternbergia; Sternbergia fischeriana; thidiazuron; Turkey (country); Embryonic development; Regeneration; Embryo; Vegetals; embryogenesis; Micropropagation; bulblet; In vitro; Sternbergia fischeriana; Organogenesis
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-004-3016-y

Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S. fischeriana were cultured on different nutrient media supplemented with various concentrations of plant growth regulators. Immature embryos produced higher number of bulblets than bulb scales. Large numbers of bulblets were regenerated (over 80 bulblets/explants) from immature embryos on Murashige and Skoog (MS) medium supplemented with 4 mg l super(-1) 6-benzylaminopurine (BA) and 0.25 mg l super(-1) alpha -naphthaleneacetic (NAA) or 2 mg l super(-1)2,4-dichlorophenoxyacetic acid (2,4-D) after 14 months of culture initiation. Regenerated bulblets were kept at 5 degree C for 5 weeks and then transplanted to a potting mixture.