Induction of heat shock proteins during the growth of Aedes albopictus cells

• Published in: Insect biochemistry Vol. 17; no. 1; pp. 199 - 206
• Main Authors: Carvalho, M.G.C., Rebello, M.A.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 1987
• Subjects: Aedes albopictus; cell culture; cell growth; heat; protein synthesis; shock; heat shock proteins; Aedes albopictus cells; growth cycle
• ISSN: 0020-1790
• DOI: 10.1016/0020-1790(87)90160-0

When cell cultures of Aedes albopictus growing at 28°C were transferred to 37°C heat shock proteins were induced. In cells derived either from lag or stationary phase two major proteins (82,000 and 66,000 daltons) were induced, while in cells derived from exponential phase four major proteins (90,000, 82,000, 76,000 and 66,000 daltons) were induced. In parallel with the induction of heat shock proteins we observed a strong inhibition of normal protein synthesis in cells derived from lag or stationary phase but not from cells derived from exponential phase. The treatment of these cultures with actinomycin D in any growth phase inhibits the induction of heat shock proteins. When heat shocked cells were returned to 28°C the synthesis of normal cellular proteins was recovered. However during recovery the cells derived from lag or stationary phase also synthesize the proteins of 90,000 and 76,000 daltons.