Differential pulse voltammetric determination of nimesulide in pharmaceutical formulation and human serum at glassy carbon electrode modified by cysteic acid/CNTs based on electrochemical oxidation of l-cysteine

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 42; no. 2; pp. 237 - 244
• Main Authors: Wang, Chengyin, Shao, Xiaoqiu, Liu, Qingxiu, Qu, Qishu, Yang, Gongjun, Hu, Xiaoya
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18.09.2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbon nanotube; Cysteic acid; Fundamental and applied biological sciences. Psychology; General pharmacology; Human serum; l-cysteine; Medical sciences; Nimesulide; Pharmacology. Drug treatments; Voltammetry; Carbon nanotube; Human serum; Cysteic acid; Voltammetry; l-cysteine; Nimesulide; Prostaglandin-endoperoxide synthase; Biological fluid; Cysteine; Determination; Electrodes; L-cysteine; Oxidation; Electrochemical reaction; Quantitative analysis; Modified material; Human; Thiol; Enzyme; Differential impulse voltamperometry; Enzyme inhibitor; Metabolism; Carbon; Sulfur containing aminoacid; Non steroidal antiinflammatory agent; Analgesic; Antipyretic; Dosage form; Oxidoreductases; Pharmacokinetics; Glassy state
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2006.03.038

Carbon nanotubes (CNTs) and cysteic acid based on electrochemical oxidation of l-cysteine (CySH) to form a novel composite thin film material at a glassy carbon electrode (GCE) for electroanalytical determination of nimesulide. The determination of nimesulide at the composite modified electrode with strong accumulation of nimesulide was studied by differential pulse voltammetry (DPV). The peak current obtained at +1.251 V (versus SCE) from DPV was linearly dependent on the nimesulide concentration in the range of 1.0 × 10 −7–1.0 × 10 −5 M in 0.05 M H 2SO 4 solution with a correlation coefficient of 0.997. The detection limit (S/N = 3) was found to be 5.0 × 10 −8 M. The low-cost modified electrode showed good sensitivity, selectivity, stability and had been applied to the determination of nimesulide in pharmaceutical formulation and human serum samples with satisfactory results.